Triphosphopyridine Nucleotide Diaphorase from Wheat Germ.

Studies on an Enzyme System from Wheat Germ Catalyzing the Aerobic Oxidation of Reduced Triphosphopyridine Nucleotide.

A triphosphopyridine nucleotide dependent alcohol dehydrogenase from Leuconostoc mesenteroides.

Ethanol formation during glucose fermentation is a relatively common activity of microorganisms; a few species, including Leuconostoc mesenterodes, produce ethanol as a major fermentation end product. The significant results from experiments with various species on this subject are: (1) at least three different metabolic pathways are available for anaerobic conversion of glucose to ethanol (Kos...

The Histochemical Localization of Triphosphopyridine Nucleotide Diaphorase

A histochemical method is described for the localization of triphosphopyridine nucleotide diaphorase using a recently synthesized tetrazolium salt (Nitro-BT). By virtue of the favorable histochemical properties of this reagent, it has been possible to demonstrate that whereas DPN diaphorase is usually restricted to the mitochondria, the TPN diaphorase activity of corresponding cells was distrib...

Nicotinic acid metabolism. 3. Purification and properties of a nicotinic acid hydroxylase.

An enzyme that catalyzes the reversible hpdroxylation of nicotinic acid to 6-hydroxynicotinic acid has been purified from extracts of a nicotinic acid-fermenting clostridium. The enzyme appears to be a flavin adenine dinucleotidecontaining non-heme iron protein and utilizes triphosphopyridine nucleotide as the ultimate electron acceptor. The purified enzyme also exhibits reduced triphosphopyrid...

The Cytochemical Localization of Oxidative Enzymes

Methods are presented for the intramitochondrial localization of various diphosphopyridine nucleotide and triphosphopyridine nucleotide-linked dehydrogenases in tissue sections. The cytochemical reactions studied involve the oxidation of the substrates by a specific pyridino-protein. The electron transfer of tetrazolium salt is mediated by the diaphorase system associated with the dehydrogenase...